ToolScript MMLV RT Kit

ToolScript RT Kit is designed for the first strand cDNA synthesis from very tiny amount of total RNA or poly (A) +RNA for two-step RT-PCR. The kit is applied combined with PCR in detection of expression of rare genes, quantitative expression level of mRNA in extremely small number of cells and cloning the cDNA of the specific gene.

 

 

No. Size Price Qty
TGKRA04 100 rxns. $305.56
The price does not include shipping fee and tax. ORDER
Features
  • Full-length fist strand cDNA up to 13kb.
  • Optimum reaction temperature 42°C
  • Complete kit: all the components for the RT reaction are included.

  

  1. Chu YD, Lin WR, Lin YH, Kuo WH, Tseng CJ, Lim SN, Huang YL, Huang SC, Wu TJ, Lin KH, Yeh CT. COX5B-Mediated Bioenergetic Alteration Regulates Tumor Growth and Migration by Modulating AMPK-UHMK1-ERK Cascade in Hepatoma. Cancers (Basel). 2020 Jun 22;12(6):1646. 

  2. Li CJ, Lin HY, Ko CJ, Lai JC, Chu PY. A Novel Biomarker Driving Poor-Prognosis Liver Cancer: Overexpression of the Mitochondrial Calcium Gatekeepers. Biomedicines. 2020 Oct 24;8(11):E451. 

  3. Wang WH, Yeh CS, Lin CY, Yuan RY, Urbina AN, Lu PL, Chen YH, Chen YA, Liu FT, Wang SF. Amino Acid Deletions in p6Gag Domain of HIV-1 CRF07_BC Ameliorate Galectin-3 Mediated Enhancement in Viral Budding. Int J Mol Sci. 2020 Apr 21;21(8):2910.

  4. Chen CY, Chen CC, Chuang WY, Leu YL, Ueng SH, Hsueh C, Yeh CT, Wang TH. Hydroxygenkwanin Inhibits Class I HDAC Expression and Synergistically Enhances the Antitumor Activity of Sorafenib in Liver Cancer Cells. Front Oncol. 2020 Feb 25;10:216. 

  5. Chu YD, Lai HY, Pai LM, Huang YH, Lin YH, Liang KH, Yeh CT. The methionine salvage pathway-involving ADI1 inhibits hepatoma growth by epigenetically altering genes expression via elevating S-adenosylmethionine. Cell Death Dis. 2019 Mar 11;10(3):240. 

  6. Huang JP, Hsu SC, Meir YJ, Hsieh PS, Chang CC, Chen KH, Chen JK, Hung LM. Role of dysfunctional adipocytes in cholesterol-induced nonobese metabolic syndrome. J Mol Endocrinol. 2018 May;60(4):307-321. 

  7. Chen CY, Fang JY, Chen CC, Chuang WY, Leu YL, Ueng SH, Wei LS, Cheng SF, Hsueh C, Wang TH. 2-O-Methylmagnolol, a Magnolol Derivative, Suppresses Hepatocellular Carcinoma Progression via Inhibiting Class I Histone Deacetylase Expression. Front Oncol. 2020 Aug 11;10:1319.

  8. Li CJ, Lin LT, Chu PY, Chiang AJ, Tsai HW, Chiu YH, Huang MS, Wen ZH, Tsui KH. Identification of Novel Biomarkers and Candidate Drug in Ovarian Cancer. J Pers Med. 2021 Apr 19;11(4):316.

  9. Li CJ, Chiu YH, Chang C, Chang YI, Sheu JJ, Chiang AJ. Acetyl Coenzyme A Synthase 2 Acts as a Prognostic Biomarker Associated with Immune Infiltration in Cervical Squamous Cell Carcinoma. Cancers (Basel). 2021 Jun 22;13(13):3125.

  10. Lin YH, Wu MH, Liu YC, Lyu PC, Yeh CT, Lin KH. LINC01348 suppresses hepatocellular carcinoma metastasis through inhibition of SF3B3-mediated EZH2 pre-mRNA splicing. Oncogene. 2021 Jul;40(28):4675-4685.

  11. ​Li CJ, Lin LT, Tsui KH. Dehydroepiandrosterone Shifts Energy Metabolism to Increase Mitochondrial Biogenesis in Female Fertility with Advancing Age. Nutrients. 2021 Jul 17;13(7):2449.

  12. ​Chen CC, Chen CY, Cheng SF, Shieh TM, Leu YL, Chuang WY, Liu KT, Ueng SH, Shih YH, Chou LF, Wang TH. Hydroxygenkwanin Increases the Sensitivity of Liver Cancer Cells to Chemotherapy by Inhibiting DNA Damage Response in Mouse Xenograft Models. Int J Mol Sci. 2021 Sep 9;22(18):9766.     
  13. Wen CY, Tsui KH, Chang CH, Chiu YH, Lin SA, Chu CY, Li CJ. Integrative Multiomics Evaluation of IIDH1 Metabolic Enzyme as a Candidate Oncogene That is Correlated with Poor Prognosis and Immune Infiltration in Prostate Adenocarcinoma. J Oncol. 2022 Jan 29;2022:9854788.

  14. Chu PY, Tzeng YT, Tsui KH, Chu CY, Li CJ. Downregulation of ATP binding cassette subfamily a member 10 acts as a prognostic factor associated with immune infiltration in breast cancer. Aging (Albany NY). 2022 Mar 5;14(5):2252-2267 
  15. Yeh TH, Liu HF, Chiu CC, Cheng ML, Huang GJ, Huang YC, Liu YC, Huang YZ, Lu CS, Chen YC, Chen HY, Cheng YC. PLA2G6 mutations cause motor dysfunction phenotypes of young-onset dystonia-parkinsonism type 14 and can be relieved by DHA treatment in animal models. Exp Neurol. 2021 Dec;346:113863.